Anti Fibronectin (bovine)
Mouse monoclonal antibody
Cat.No. CSI 005-32
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities (1).
Bovine corneal endothelial cells
CSI 005-32 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin).
CSI 005-32 cross-reacts with human fibronectin. Other species have not been tested.
Epitope is located in the 40kD Hep II heparin-binding domain, but differs from that of CSI 005-35
CSI 005-32 can be used in ELISA, Western blotting, immunoprecipitation and immunostaining of frozen PLP-fixed sections of bovine and human tissues. The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain. It can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA with antibody CSI 005-35.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:4000 | 1,2,3,4 |
| Immunoblotting | Yes | 1:100 | 1 |
| Immunohistochemistry | Yes |
1. Underwood PA, Dalton BA, Steele JG, Bennett FA, Strike P (1992) Anti-fibronectin antibodies that modify heparin binding and cell adhesion: evidence for a new cell binding site in the heparin binding region. J Cell Sci 102:833-845.
2. Underwood PA, Steele JG, Dalton BA (1993) Effects of polystyrene surface chemistry on biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies. J Cell Sci 104:793-803.
3. Di Girolamo N, Underwood PA, McCluskey PJ, Wakefield D (1993) Functional activity of plasma fibronectin in patients with Diabetes mellitis. Diabetes 42:1606-1613.
4. Dalton BA, McFarland CD, Underwood PA, Steele JG (1995) Role of heparin binding domain of fibronectin in attachment and spreading of human bone derived cells. J Cell Sci 108:2083-2092.
Downloads
CSI 005-32.pdf (12.46 kB) Product specification
Cell biology flyer.pdf (197.81 kB)
Cell biology flyer
Related Articles
Antibody MSDS
April 23 2008
